Upcoming Conference to Discuss DNA Sequencing for Detecting Food Microorganisms

March 25, 2011

The event’s overall goal is to address knowledge gaps regarding molecular detection and subtying of food-borne pathogens and spoilage organisms.

The upcoming 4th annual Molecular Methods in Food Microbiology Symposium will focus on the use of DNA sequencing, including conventional Sanger and next-generation sequencing, for the molecular detection and subtyping of food-associated microorganisms. The event’s overall goal is to address and fill-in knowledge gaps regarding molecular detection and subtying of food-borne pathogens and spoilage organisms for current and future food-safety professionals.

The event will be held June 27-July 1 at Colorado State University in Fort Collins. Together with Colorado State University, it is being presented by Cornell University, Purdue University, and testing company Silliker Inc. (Chicago).

The program will comprise:

A two-and-a-half day hands-on laboratory session entailing 16S rDNA sequence-based identification, multilocus sequence typing, design of a custom multiplex detection method for STEC or other targets of interest, and application of commercially available multiplex real-time PCR assays to detect foodborne pathogens.

A half-day discussion and evaluation of commercially available assays for the detection and subsequent characterization of non-O157 shiga toxin encoding Escherichia coli (STEC).