Vitamin E: A Closer Look at Tocotrienols: Page 2 of 2

Oct 17, 2017

Tocotrienols’ Time to Shine

While alpha-tocopherol research still occurs at a much greater level than tocotrienol research, there has been a downward trend in alpha-tocopherol research alongside an increase in understanding of tocotrienols driven by a growing body of science. Furthermore, FDA’s impending changes to the U.S. Nutrition Facts label, which include changing the unit of measure for vitamin E from IU to mg, will further underscore the differences between the two forms. Under the new labeling standard, 1 mg of natural vitamin E (RRR-alpha-tocopherol) equals 1 mg of vitamin E, but 2 mg of synthetic vitamin E (all-rac-alpha-tocopherol) equates to only 1 mg of natural vitamin E. The reason? In a nutshell: when synthesizing alpha-tocopherol, the stereochemistry changes that occur result in molecules that are not identical to natural alpha-tocopherol, although they may still be of the same molecular weight.  

Growing consumer demands for natural sources of vitamin E, coupled with promising research in several important health categories, should fuel more interest in tocotrienols. Below are just a few study areas of interest showing positive results. 


Editor’s note: Asterisks indicate studies performed by the author’s company, American River Nutrition (Hadley, MA), or on the company’s DeltaGold annatto tocotrienol ingredient. Some studies were also funded by American River Nutrition.


Cardiovascular Health

Thirty-one subjects with high cholesterol took escalating doses of tocotrienols—125, 250, 500, and 750 mg/day of American River Nutrition’s DeltaGold annatto tocotrienol—for four weeks each, with a two-week wash-out period between each dose. Researchers concluded that total cholesterol fell by 15%, while LDL cholesterol was reduced by 18%. Other notable outcomes: triglycerides decreased by 14%, and the cytokines associated with cardiovascular disease decreased by as much as 64%.17 *



The same parameters were employed to examine DeltaGold annatto tocotrienols’ effect on inflammatory markers, with equally impressive results. (Note: In this study and the one previously profiled, 250 mg/day was identified as an optimal dose of tocotrienols.) Among the promising results: C-reactive protein levels (a predictor of chronic inflammation) were reduced by 40%, and nitric oxide levels, which are aberrant in inflammatory conditions and should be reduced (only in inflammatory conditions), were also lowered by 40% in subjects.23

Two clinical studies indicate that delta-tocotrienol, combined with antioxidant polyphenols, may curb inflammation and help manage dyslipidemia (the elevation of plasma cholesterol), triglycerides, or both, or help to lower high-density lipoprotein levels that contribute to the development of atherosclerosis.24,25 * One placebo-controlled study was conducted in two groups of elderly subjects over six weeks, one with normal lipid levels, the other elevated. The product formulation was composed of DeltaGold delta-tocotrienol from annatto along with a B-vitamin (niacin) and polyphenols. In both groups, supplementation led to a significant drop in C-reactive protein and -glutamyl-transferase (a predictor of non-fatal myocardial infarction and fatal coronary heart disease), while increasing total antioxidant status. In the hypercholesterolemic group, the following also dropped: LDL cholesterol (by 20%-28%) and triglycerides (by 11%-18%). C-reactive protein dropped in healthy elderly subjects (by 21%-29%) and in the hypercholesterolemic elderly (by 31%-48%). No adverse effects were observed.


Bone Health

In a 2017 in vivo study involving 48 female Sprague-Dawley rats, DeltaGold tocotrienol from annatto—with or without lovastatin—increased bone formation and mineralization in rats. Researchers concluded that “supplementation of tocotrienol in statin users can potentially protect them from osteoporosis.”26

Our company is also embarking on (and sponsoring) a study of DeltaGold tocotrienols’ effect on the bone health of 89 postmenopausal women on tocopherol-free tocotrienol. This study will hopefully provide welcome clarity on how the nutrient affects human subjects. The study should be published in late 2017. As of this writing, early results look very promising. 



Tocotrienols’ bioavailability has been questioned in the past, resulting in the marketing of bio-enhanced, synthetic emulsions that are said to increase absorption. The solution for increasing tocotrienols’ bioavailability is easy, however, and time-tested: take tocotrienols with a meal. 

Two studies27,28 on DeltaGold confirmed the bioavailability of tocopherol-free annatto tocotrienol. An open-label randomized trial involved 125, 250, 500, 750, and 1000 mg/day dosage groups. Many pharmacokinetic parameters were studied in healthy subjects to ascertain that delta- and gamma-tocotrienol were absorbed. When taken with a meal, both tocotrienols were absorbed and bioavailable.*


The Future for Tocotrienols

Research clearly shows that not all vitamin E isomers are the same. Tocotrienols have well-differentiated functions from tocopherols, with a growing body of evidence supporting its benefits for chronic conditions. Considering that studies show that tocopherol does not augment the function of tocotrienol, but rather antagonizes it, in my professional opinion, in order to fully appreciate the benefits of tocotrienol, manufacturers should formulate with delta- and gamma-tocotrienols, without tocopherol or with the lowest amount of alpha-tocopherol possible. 


Barrie Tan, PhD, a foremost vitamin E expert, is credited with identifying the primary sources of plant-based tocotrienols. Today, his research focuses on nutrients that impact aging conditions. Tan is the senior editor of Tocotrienols: Vitamin E Beyond Tocopherols (2013), and collaborates with universities worldwide to further tocotrienol research.



Also read: 

Tocotrienols May Promote Bone Health in Postmenopausal Osteopenic Women in New Study

Tocotrienol Regulates Gastric Growth Factors Better than Omeprazole in Animal Study

Vitamin E Research Ramps Up

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  14. Qureshi AA et al., “Tocopherol attenuates the impact of gamma-tocotrienol on HMG-CoA reductase activity in chickens,” Journal of Nutrition, vol. 126, no. 2 (February 1996): 389-394
  15. Khor HT et al., “Effects of administration of alpha-tocopherol and tocotrienols on serum lipids and liver HMG CoA reductase activity,” International Journal of Food Sciences and Nutrition, vol. 51, suppl. 1 (2000): S3-S11
  16. Heng KS et al., “Potential of mixed tocotrienol supplementation to reduce cholesterol and cytokine levels in adults with metabolic syndrome,” Malaysian Journal of Nutrition, vol. 21, no. 2 (2015): 231-243
  17. Qureshi AA et al., “Dose-dependent modulation of lipid parameters, cytokines and RNA by delta-tocotrienols in hypercholesterolemic subjects restricted to AHA step-1 diet,” British Journal of Medicine & Medical Research, vol. 6, no. 4 (2015): 351-366
  18. Peralta EA et al., “Vitamin E increases biomarkers of estrogen stimulation when taken with Tamoxifen,” The Journal of Surgical Research, vol. 153, no. 1 (May 2009): 143-147 
  19. Khanna S et al., “Excessive -tocopherol exacerbates microglial activation and brain injury caused by acute ischemic stroke,” The FASEB Journal, vol. 29, no. 3 (March 2015): 828-836 
  20. Khanna S et al., “Delivery of orally supplemented alpha-tocotrienol to vital organs of rats and tocopherol-transport protein deficient mice,” Free Radical Biology & Medicine, vol. 39, no. 10 (November 15, 2005): 1310–1319
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  22. Ikeda S, Uchida T, Abe C. Bioavailability of Tocotrienols and Interference of Their Bioavailability by Alpha-Tocopherol Supplementation. In: Tocotrienols: Vitamin E Beyond Tocopherols, 2nd ed. Tan B, Watson R, Preedy V, editors. Boca Raton, FL: CRC Press; 2013: 53-60
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  24. Qureshi AA et al., “Suppression of nitric oxide production and cardiovascular risk factors in healthy seniors and hypercholesterolemic subjects by a combination of polyphenols and vitamins,” Journal of Clinical & Experimental Cardiology. Published online June 7, 2012.
  25. Qureshi AA et al., “Nutritional supplement-5 with a combination of proteasome inhibitors (resveratrol, quercetin, -tocotrienol) modulate age-associated biomarkers and cardiovascular lipid parameters in human subjects,” Journal of Clinical & Experimental Cardiology. Published online March 2, 2013.
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  28. Qureshi AA et al., “Evaluation of pharmacokinetics, and bioavailability of higher doses of tocotrienols in healthy fed humans,” Journal of Clinical & Experimental Cardiology. Published online April 28, 2016.